(LML) request for advice research detection of cytokines

Leprosy Mailing List – July 14,  2016

Ref.:   (LML) request for advice research detection of cytokines

From:  Jingquan wang, Zhejiang, China

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Dear Pieter,

 

Greetings from Zhejiang, China.

 

I intend to make a research related to the detection of the cytokines including IL-17, cell transcription factor ROR yt  by Th 17  and Foxp3 and IL-10 by T regulatory cell  in the skin lesions in leprosy patients, by the method of immunohistochemistry. But I have not found some paper related to the measurement of cytokines in the paraffin-embedded samples with immunohistochemistry among leprosy patients. They usually detected IL-17 in the serum by flow cytometry. I wonder if anyone detect the IL-17 in the skin lesions by immunohistochemistry? Is the detection method available now? Why there was no report related to the cytokine detection by immunohistochemistry? Is the level of IL-17,IL 10,et al  in the skin lesions same with those in the serum in leprosy patients? If anyone in LML have some papers related to it, I would like to read them.

 

Thanks in advance and welcome any suggestions.

 

Best regards,

 

Jingquan WANG
Bachelor of Medicine(MB),Chief Physician

Institute of Dermatology of Zhejiang Province,

No. 61,Wuyuan Street,Wukang, Deqing,313200,

Zhejiang,P.R.China

Under-Secretary-General of China Leprosy Association

Tel:86-572-8296002

Fax:86-572-8078115

E-mail:wjingquan@126.com  767790635@qq.com

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