Leprosy Mailing List – July 29, 2014
Ref.: (LML) Staining or PCR detection of M. leprae
From: Norihisa Ishii and Koichi Suzuki, Tokyo, Japan
Dear all,
We noticed that some technical issues are occasionally discussed in LML, such as the staining or the PCR detection of M. leprae. We would like to share some information about the laboratory diagnosis of leprosy that is performed in our lab at the Leprosy Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
We are routinely performing the following examinations:
- histopathological staining of paraffin sections (H&E, Fite staining, S100 immunostaining, and others if necessary)
- staining of acid-fast bacilli using a slit-skin smear samples on a glass slide
- detection of serum PGL-I antibody titers
- PCR detection of M. leprae genes from slit-skin smear, tissues or paraffin sections
In Japan, we are responsible to perform the above tests for differential diagnosis of leprosy as a part of government service with free of charge. We are willing to provide the above laboratory examinations for samples from all the countries if they are sent appropriately. Please consult Dr. Norihisa Ishii or Dr. Koichi Suzuki for further inquiries.
With kind regards,
Norihisa Ishii (norishii@nih.go.jp)
Koichi Suzuki (koichis@nih.go.jp)
LML - S Deepak, B Naafs, S Noto and P Schreuder
LML blog link: http://leprosymailinglist.blogspot.it/
Contact: Dr Pieter Schreuder << editorlml@gmail.com
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