Leprosy Mailing List – July 30, 2014
Ref.: (LML) Staining of M. leprae
From: P.K. Das, Birmingham, U.K.
Dear Pieter,
Thank you for the letters of David Schollard and Jaison Barreto (LML July 25, 2014).
Variable results in Fite vs ZN staining is well recognised. In order to overcome such difficulty, I wonder whether or not one should advocate the staining with specific LAM and PGL-1 antibodies, which is quite easy at least not more difficult than Fite and ZN.
In regards to Jaison’s remark, I fully sympathise with his compassion. However, in regards to the dogma that M.leprae has affinity for Schwann cells and the notion that once infected M.leprae goes to Schwann cells, I question if it is really absolute correct or does M.leprae hide away from the rising immune attack by the host and take refuge in Schwann cells in face of the immune attack.
My experience with Patricia Rosa in ILSL, who carries out routinely mouse foot pad inoculation, is that to my knowledge we were not able to find any AFB in the nerves or Schwann cells adjacent to the foot pad site where the bacilli were multiplying.
One should carefully reconsider this dogma. Most likely David may comment on this.
Regards,
Pranab
LML - S Deepak, B Naafs, S Noto and P Schreuder
LML blog link: http://leprosymailinglist.blogspot.it/
Contact: Dr Pieter Schreuder << editorlml@gmail.com
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