Leprosy Mailing List – July 14, 2016
Ref.: (LML) request for advice research detection of cytokines
From: Jingquan wang, Zhejiang, China
Dear Pieter,
Greetings from Zhejiang, China.
I intend to make a research related to the detection of the cytokines including IL-17, cell transcription factor ROR yt by Th 17 and Foxp3 and IL-10 by T regulatory cell in the skin lesions in leprosy patients, by the method of immunohistochemistry. But I have not found some paper related to the measurement of cytokines in the paraffin-embedded samples with immunohistochemistry among leprosy patients. They usually detected IL-17 in the serum by flow cytometry. I wonder if anyone detect the IL-17 in the skin lesions by immunohistochemistry? Is the detection method available now? Why there was no report related to the cytokine detection by immunohistochemistry? Is the level of IL-17,IL 10,et al in the skin lesions same with those in the serum in leprosy patients? If anyone in LML have some papers related to it, I would like to read them.
Thanks in advance and welcome any suggestions.
Best regards,
Jingquan WANG
Bachelor of Medicine(MB),Chief Physician
Institute of Dermatology of Zhejiang Province,
No. 61,Wuyuan Street,Wukang, Deqing,313200,
Zhejiang,P.R.China
Under-Secretary-General of China Leprosy Association
Tel:86-572-8296002
Fax:86-572-8078115
E-mail:wjingquan@126.com 767790635@qq.com
LML - S Deepak, B Naafs, S Noto and P Schreuder
LML blog link: http://leprosymailinglist.blogspot.it/
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