Leprosy Mailing List – October 10th, 2011
Ref.: Choosing the decolourizer and its strength to stain Mycobacterium leprae. Does it actually matter?
From: R de Soldenhoff, Edinburgh, Scotland, UK
Dear Dr Noto,
It may be of interest to LML readers to review the attached article of an operational research study carried out in Indonesia. The original in Leprosy Review was published prior to all their articles being in electronic form, so my apologies for the mediocre quality scan of the article.
The reason for the study was the premise that "tuberculosis diagnosing centres with an established system of quality control, can also be responsible for leprosy microscopy. This is easier if the staining technique for both organisms is identical."
I would be very pleased if another laboratory could repeat the study, in order to confirm these findings. In addition, a comparison between the "hot method" (usually used in field laboratories for tuberculosis) and the "cold method" and between methylene blue and malachite green (occasionally used as an alternative decolouriser), would also be of interest.
With kind regards,
Dr. Richard de Soldenhoff
The reason for the study was the premise that "tuberculosis diagnosing centres with an established system of quality control, can also be responsible for leprosy microscopy. This is easier if the staining technique for both organisms is identical."
I would be very pleased if another laboratory could repeat the study, in order to confirm these findings. In addition, a comparison between the "hot method" (usually used in field laboratories for tuberculosis) and the "cold method" and between methylene blue and malachite green (occasionally used as an alternative decolouriser), would also be of interest.
With kind regards,
Dr. Richard de Soldenhoff
42 Drummond Place
Edinburgh, EH3 6NR
Scotland, UK
Home telephone: 44.131.556.7979
UK mobile: 44.777.0751578
Email: rdesoldenhoff(at)hotmail.com
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