Leprosy Mailing List – October 10th, 2011
Ref.: Is 0.6% fucsin reagent significantly less effective?
From: L Gilead, Jerusalem, Israel
Dear Dr. Noto,
Thank you very much to Dr Barreto for his comments (LML Oct. 2nd, 2011) concerning aspects of skin smears examination for leprosy and Ziehl-Neelsen (ZN) staining for acid-fast bacilli (AFB's). Following that message we re-evaluated our lab reagents and found out that the concentration of the Ziehl's fucsin reagent we are using (commercially acquired from MERK is 0.6%.
I would appreciate it if any one of the LML members could let us know if this is significantly less effective and we need to prepare our own reagent in the lab with a 1% concentration, or whether any one has a different commercial supplier that can deliver a 1% reagent?
Thank you very much and a Happy New Hebrew year from Jerusalem.
Leon
Dr. Leon Gilead
Head of The Israeli Hansen's Disease Center
24Strauss St., Jerusalem, Israel
Department of Dermatogoy
Hadasssah University Hospital
Mobile: 972-50-7874309
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